Which of the following is an accepted method of sterilization?

Moist heat is an accepted method of sterilization that effectively destroys microorganisms, including bacterial spores. It primarily includes techniques such as autoclaving, where steam under pressure is utilized to achieve sterilization. This method is particularly favored because it is effective at lower temperatures and is able to penetrate materials effectively, leading to a more reliable sterilization process.

The principles behind moist heat sterilization involve denaturation of proteins and destruction of nucleic acids, rendering the microorganisms inactive. Since this method is widely recognized for its efficiency and reliability in a clinical setting, it aligns with established standards for sterilization practices in healthcare and laboratory environments.

Other methods such as dry heat and cold sterilization, while they can be used in certain contexts, do not always achieve the same levels of microorganism destruction as moist heat does under standard conditions. Additionally, chemical solutions may be used for disinfection and antisepsis but are not considered a primary method of complete sterilization, particularly for instruments that must be sterile.